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KMID : 0613820090190040429
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Journal of Life Science
2009 Volume.19 No. 4 p.429 ~ p.435
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Increased Antioxidative Activities against Oxidative Stress in Saccharomyces cerevisiae KNU5377
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Kim Il-Sup
Yun Hae-Sun
Lee Oh-Seuk
Yang Ji-Young
Jin Ingn-Yol
Yoon Ho-Sung
Park Heui-Dong
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Abstract
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Oxidative stress is a consequence of an imbalance of the defense system against cellular damage generated by reactive oxygen species (ROSs) such as superoxide anions (menadione; MD). Most organisms have evolved a variety of defense systems to protect cells from adverse conditions. In order to evaluate stress tolerance against oxidative stress generating MD, comparative analyses of antioxidant capacity, or free radical scavenger ability, were performed between S. cerevisiae KNU5377 (KNU5377) and three wild-type S. cerevisiae strains. In a medium containing 0.4 mM MD, the KNU5377 strain showed higher cell viability and antioxidant ability, and contained higher levels of trehalose, superoxide dismutase, thioredoxin system, glucose-6-phosphate dehydrogenase, and some heat shock proteins. The KNU5377 strain also produced a lower level of oxidative stress biomarker than the other three yeast strains. These results indicate that S. cerevisiae KNU5377 has a higher level of tolerance to oxidative stress due to the increased expression of cell rescue proteins and molecules, thus alleviating cellular damage more efficiently than other S. cerevisiae strains.
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KEYWORD
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Saccharomyces cerevisiae KNU5377, oxidative stress, antioxidant enzymes, heat shock protein, stress tolerance
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